FastQCFastQC Report
Mon 21 Jul 2025
SCP01_MKRN250024227-1A_22VNWKLT4_L6_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP01_MKRN250024227-1A_22VNWKLT4_L6_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14683260
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGA3850.026.1812731
CGCGTCG2308.359467E-418.7789553
GGCGACT26550.018.4402941
GCCGGCT10250.018.2630351
TCTCGCA20450.017.6004897
TCCGTAC62000.017.3011572
GTCCGTA66150.016.8703981
CCGTACC66500.016.3456943
TTCTCGC20150.016.0762776
GCCGGCC4501.3172339E-615.9996671
GTCGGAT51450.015.9530211
GCGACTT28450.015.6888082
CGCGTAG3305.2711874E-415.272411
GCCGGAT14700.015.1833571
TTCCCCT74250.015.124337
GTGCAAT78150.015.1090911
TCGCATC21000.015.0828319
ACCGCGG8251.0913936E-1114.83352859
CCTAGCT18450.014.8289591
GCGCTGT3901.1145294E-414.7689231
Produced by FastQC (version 0.11.5)