FastQCFastQC Report
Mon 21 Jul 2025
SCP01_MKRN250024227-1A_22VNWKLT4_L6_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCP01_MKRN250024227-1A_22VNWKLT4_L6_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14683260
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT27450.022.8292871
CGGGCCG1600.002263016822.504883
GCGACTT27650.020.5800972
CCTAGCT15250.017.4762131
TCCGATC20550.016.8211693
GTGCAAT77150.016.3387031
TTCTCGC19300.016.0407246
TTCCCCT61650.015.9992757
CTATGCG22150.015.9270438
TCCGTAC50100.015.6712712
TCTCGCA19450.015.5468537
CCGTACC51750.015.1685083
TCGCATC20000.014.7593319
GCGGGCC2950.00439468214.6502392
GTCCGTA55450.014.2891571
GCCACAC83050.014.2239311
GCGAAAT34750.014.0951451
GTCGGAT43200.014.0058991
GCGGCAT27850.012.9318391
CGCGAAT3950.00204400612.7648711
Produced by FastQC (version 0.11.5)