FastQCFastQC Report
Mon 21 Jul 2025
SCE03_MKRN250024226-1A_22VTJNLT4_L5_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE03_MKRN250024226-1A_22VTJNLT4_L5_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7159795
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT5858.3255145E-814.769831
GTCGGAT24500.014.6944731
CCGGGAT9650.014.1767411
GTCGCGA3050.00549662614.1645091
GCCGGGT4905.188358E-513.2250251
GCCGGAT10706.184564E-1112.112641
GTCCGGA4907.674171E-411.7555781
TAGCGTG17550.011.4866828
TCGCATC7301.5873122E-510.8489972
GTCGGGA7552.3013432E-510.4904921
GGCGACT12801.3951649E-910.1254111
GGCGAAC5700.002710319410.1056731
GGCGGCT6507.1619125E-49.9696351
GGCGAAT18850.09.93143751
GCCGTCT8701.0054029E-59.9314371
GCGAGAT8801.1515922E-59.818581
CGGATTC37450.09.8046493
CCCGTAT11051.7541242E-79.7741511
GCCGTTT21000.09.6003891
GCGGCAT15303.1650416E-109.4121471
Produced by FastQC (version 0.11.5)