FastQCFastQC Report
Mon 21 Jul 2025
SCE03_MKRN250024226-1A_22VTJNLT4_L5_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE03_MKRN250024226-1A_22VTJNLT4_L5_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7159795
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGAT9902.1282176E-1012.3699121
TCCTCGC4806.461295E-411.998887
CATCGCG4200.003251059411.9988799
GTCGGAT24250.011.8823171
GCCGGCT5953.1559926E-410.8962841
GCGAGAT6254.9709214E-410.3732621
TAGCGTG17200.010.0455748
GGCGACT11854.62187E-89.7264541
CGGATTC31050.09.2750163
GGAAAAT150650.08.89399051
GTCCGTA6550.0084897888.7983571
GCCGAAT20801.8189894E-128.6582381
CCCGGTT10005.1747833E-58.6443851
AGCGTGA19303.8198777E-118.5795119
GGCAAAT92550.08.561881
ATCCGGC8900.00136809288.0891336
GGGGGAT18851.8608262E-98.02529051
GCGACAT22158.731149E-117.8053141
GCGATGT9350.00221068857.70444251
GTCGAGG12256.535237E-57.64469431
Produced by FastQC (version 0.11.5)