FastQCFastQC Report
Mon 21 Jul 2025
SCE03_MKRN250024226-1A_22VNWKLT4_L6_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE03_MKRN250024226-1A_22VNWKLT4_L6_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13522241
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGC4151.0838201E-515.6134521
GCGGGGT5354.889971E-714.8027841
GCGACGT9105.2750693E-1113.4496831
GCGCACT3800.001533025213.2623031
GCCGGCT12100.013.0900671
GTCGGAT48950.012.94298651
GTCCGCA5902.1719612E-512.2026041
GCCGGGA13400.011.8201331
GGCGCCT5551.6635793E-411.6749241
GCCCGGT6203.6082336E-511.6121551
CTCGCCG5100.001074085211.293391
GGCGAAT34000.011.2228061
GCGGGAC4500.00544124411.1992781
GCGAGAC7251.4718358E-510.9234341
GCCCGCC4650.00694445710.8380121
GCCGTGT16650.010.3777091
CGCAGTC8608.787818E-610.0446763
GCGACAT45750.09.9141151
GGCGTTT36200.09.7452291
GGCGACT21550.09.6884711
Produced by FastQC (version 0.11.5)