FastQCFastQC Report
Mon 21 Jul 2025
SCE03_MKRN250024226-1A_22VNWKLT4_L6_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE03_MKRN250024226-1A_22VNWKLT4_L6_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13522241
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT42450.011.70819951
GCGGAAT18900.011.0523571
CGGATCG8204.956297E-610.5389213
GGCGGCT12458.56744E-1010.41410351
GCGAGCT7352.0174737E-49.800121
GCCCGGT6800.00107936349.5334991
CTCACGT30350.09.4889048
GGCGACT20900.09.3054251
GGCGAAT34700.09.1335991
GGCAAAT181450.09.0113041
TAGCGTG29800.08.9392328
GCGCATT8105.343632E-48.8927011
GCCGGAT19153.274181E-118.6512291
GCGGAAG21003.637979E-128.5751061
TCCTCGC9302.2495577E-48.5158136
CCTCGCA10156.2011306E-58.5119987
CCGGAAT51550.08.2440781
CCTAGCT12257.360606E-68.23210051
CGCGAAG8000.0047179548.1034741
CTCGGAT18107.417839E-97.9592141
Produced by FastQC (version 0.11.5)