FastQCFastQC Report
Mon 21 Jul 2025
SCE02_MKRN250024225-1A_22VTJNLT4_L5_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE02_MKRN250024225-1A_22VTJNLT4_L5_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15042968
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT53950.015.6163491
CCCGGCC4201.21194535E-515.430441
AGCGCTC4252.3125537E-413.5527372
GCCGGCT12350.013.4105311
GTTCGGG15000.012.961571
GCGGACT3900.001861514412.9246431
GGCGACT23200.011.7945321
GCCCGGT6153.3154658E-511.7087361
CTCGCAT9852.6066118E-911.6968491
CCGGCCC4550.005905712511.07675652
GCGGAAT23150.010.88684751
CGGATTC77850.010.6353943
TCGGATT91150.010.584592
GCTCGCT5750.00291220110.0186051
TAGCGTG33250.09.7439218
CCGGAAT57150.09.7019631
GCCGGGT9001.5022182E-59.6011631
GCGGTAT24150.09.5415291
CGGGAAT36750.09.4052211
GCGACTT23150.09.3303122
Produced by FastQC (version 0.11.5)