FastQCFastQC Report
Mon 21 Jul 2025
SCE02_MKRN250024225-1A_22VTJNLT4_L5_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE02_MKRN250024225-1A_22VTJNLT4_L5_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15042968
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT49850.014.7383291
GTCGGCT18550.012.0373541
GTCGGAC10254.8457878E-911.2437041
CTCACGT29550.010.9634538
GCCGGAT24250.010.69310951
GCGCTCC4750.00810396510.61494351
GCGACAT46200.010.2899971
GCGCATT9251.8864339E-610.1230982
TCGGATT83100.09.62132
GGCGCTT8407.400429E-59.4324961
GGCGAAT36050.09.3908711
GCGGAAT22400.09.325311
GCGCAAT13503.5444828E-89.0704411
GGCGCAG7300.00206145718.8804081
CGGATTC70750.08.7516013
TAGCGTG30450.08.747968
CCTAGCT17353.6743586E-108.7183261
GGCGCAT8306.814829E-48.678311
GGCAAAT192650.08.5620891
CCGGCAT44800.08.5214031
Produced by FastQC (version 0.11.5)