FastQCFastQC Report
Mon 21 Jul 2025
SCE02_MKRN250024225-1A_22VNWKLT4_L6_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE02_MKRN250024225-1A_22VNWKLT4_L6_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15769441
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT56850.018.7443871
GCCGGCT13250.012.4983281
CTCGCGA5209.055311E-512.4617541
CGGATTC82400.011.70649053
CGCGTAT5601.8069573E-411.5716291
TCGGATT96000.011.3984252
CGCGTGT4450.005003577611.3260381
CGCGGTG5200.00126257111.0771141
GTCGCGA4650.00694032910.8388971
GCGGAAC21100.010.2371441
GCGCACT5650.002520053610.1948671
GTCGGAC11653.5804987E-89.8885831
GGCGCAT8751.0768061E-59.8744561
GGCGACT25850.09.4701831
GCGGAAT22950.09.4119271
GGCGAAT41200.09.2622971
CCTAGCT18009.094947E-129.2001591
GGCGGCT13553.7882273E-89.0333661
GCGAATC23950.09.0175442
CTCGGAG10408.433093E-69.0001551
Produced by FastQC (version 0.11.5)