FastQCFastQC Report
Mon 21 Jul 2025
SCE02_MKRN250024225-1A_22VNWKLT4_L6_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE02_MKRN250024225-1A_22VNWKLT4_L6_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15769441
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT3754.1154617E-617.2881581
CCGCGTT4151.8820452E-413.8860711
GTCGGAT52900.012.3914811
GGCGAAT41000.011.4200231
TAGCGTG35850.010.2422178
CCGGAAT60050.010.1963191
CCTAGCT17000.010.1695051
GCCGTAT20300.09.9357231
GGCGACT24900.09.5466741
GCCGAAT42350.09.5251561
TCCTCGC10601.0470212E-69.50900656
CTCACGT32850.09.2050668
GGCAAAT204650.09.0108491
GCCGGCT16952.3101165E-108.9245651
CGCGTTC6550.0084931888.7980452
GCGGCAT32650.08.6043661
GGAAAAT325950.08.5304871
TCGGCCG7600.00298257038.5259337
CGGGAAT36400.08.509511
CCTCGCA11905.0178205E-68.4702077
Produced by FastQC (version 0.11.5)