FastQCFastQC Report
Mon 21 Jul 2025
SCE01_MKRN250024224-1A_22VTJNLT4_L5_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE01_MKRN250024224-1A_22VTJNLT4_L5_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6599388
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT4058.8039087E-1021.335461
GTCCGCT3403.446087E-516.9428671
GGCGACT11650.015.4521851
GCCAGGA8853.274181E-1113.8318881
CCCGGTT8252.3519533E-913.0922151
GTCGGAT22000.012.4376051
GCGACTT11301.2732926E-1112.1059352
GGCGGAT9601.7389539E-912.0011971
GCGCAAT6203.6015335E-511.6140611
GCCGGCT6857.82254E-611.5631981
GCGAGAT7652.1593733E-611.2952441
GTCCGAG4500.00543409411.20111751
GCGTGAG19750.010.5719252
GCGAACT4850.00947850410.3927891
GTCGGAA15950.010.383481
GCCGGAT10508.3166015E-810.286741
GGCGGAG8507.618073E-610.1657211
CCCGTAT11452.7332135E-810.06213951
CCGGAAT23950.09.6210021
CTCACGT14309.295036E-109.5660118
Produced by FastQC (version 0.11.5)