FastQCFastQC Report
Mon 21 Jul 2025
SCE01_MKRN250024224-1A_22VTJNLT4_L5_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE01_MKRN250024224-1A_22VTJNLT4_L5_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6599388
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT1750.003802254320.5792391
GCGAACT4653.1610907E-513.9407761
GGCGACT10001.8189894E-1112.9649221
GGCGAAT16000.012.6047841
GTCGGAT20800.012.1199851
GCGGGAG4200.003239524612.0045561
GCGCAAT5651.9556553E-411.4733811
GCGGGAT5200.001258585511.0811291
CGGGAAT14350.011.042521
GCGACTT9302.021472E-610.0683381
GCCGAAT19500.09.9730171
GCGAGAT7301.8840017E-49.866761
GCGAAGT6709.427569E-49.6753141
CCGGGAT8951.4015655E-59.6572971
CTCACGT13956.076516E-88.7734028
GCGAATT17554.6020432E-108.6186562
GGAAAAT140900.08.5369531
GGCAAAT83800.08.4232451
GCTCGAT12509.652429E-68.0670621
TAGCGTG16107.078961E-88.04896458
Produced by FastQC (version 0.11.5)