FastQCFastQC Report
Mon 21 Jul 2025
SCE01_MKRN250024224-1A_22VNWKLT4_L6_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE01_MKRN250024224-1A_22VNWKLT4_L6_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17891069
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT16050.014.8036241
GCGCGAT3457.384382E-414.60858251
GCCCGGT7055.6219505E-813.2764931
CGCGTGA5801.8216006E-512.4136961
GCGGGAT18650.012.3537911
GTCGGAT62550.012.3164511
GGCGGCT17650.012.2378651
GCCCGGA6304.246217E-511.4284831
GGCGCAC7009.967091E-611.3141981
GCCCGCT5100.001073605311.294031
GCGCACT5200.001262850411.0768371
CGCGTGT4600.006405669310.9564371
GCGGACT4700.00751559710.7233211
GCGAGAT17650.010.1982211
GCCCGCC5650.002520607310.1946111
GGCGCTT8608.770052E-610.0464331
GGCGACT29700.09.9393171
GCGGAAT26100.09.9309581
GCCTAGC14258.869392E-99.09466651
GGGAGCG7200.00182855788.9982964
Produced by FastQC (version 0.11.5)