FastQCFastQC Report
Mon 21 Jul 2025
SCE01_MKRN250024224-1A_22VNWKLT4_L6_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSCE01_MKRN250024224-1A_22VNWKLT4_L6_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17891069
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT57650.014.1199131
CGGATCG10653.637979E-1212.8481773
AGCGCTC5009.056505E-411.5258472
TAGCGTG40000.010.7995328
CCTAGCT16750.010.7517231
GGCGAAT43200.010.505331
GGCGACT29100.09.9019311
GCCGAAT51350.09.2588351
GCGGGAT18751.8189894E-129.2206781
GCGACAT56350.09.2043151
GCGCTCC6350.00659851879.073063
CTCACGT37000.08.9509648
CGGGAAT42600.08.7931941
GGCAAAT231950.08.5717121
GGAAAAT381600.08.532631
CGGTTCG11854.7231424E-68.5083873
CTCGAAT54250.08.4983211
CTCGCAT12206.9606594E-68.2664891
TCGTAGC18401.1732482E-98.2170817
CCGCAAT30700.08.2126361
Produced by FastQC (version 0.11.5)